Officer O'Brien engaged in an on-duty confrontation with four drunken men on Marshall Street between Hancock and Clay Streets. One of the men, Frank Kleber, pulled a pistol and fatally shot Officer O'Brien in the abdomen. Officer O'Brien managed to return fire and hit Kleber twice. Kleber survived the shooting.
Frank Kleber and one of the other four men were charged in Officer O'Brien's death. Kleber was charged with murder but was found guilty in trial of a lesser crime. He was sentenced to three years in the penitentiary. An accessory to murder charge against the other individual was dismissed.
Officer O'Brien served with the Louisville Division of Police from 1895 until his death.
There are currently no tributes for Patrolman John T. O'Brien.